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Targeting herpes simplex virus with CRISPR-Cas9 cures herpetic stromal keratitis in mice.

Yin D, Ling S, Wang D, Dai Y, Jiang H, Zhou X, Paludan SR, Hong J, Cai Y
Nat Biotechnol. 2021 Jan 11. pii: 10.1038/s41587-020-00781-8. doi: 10.1038/s41587-020-00781-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
166685pLV-U6-UL29-U3-UL8To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).
166694pLV-U6-UL29-egfp-U3-UL8To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

Antibodies from Article