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tTARGIT AAVs mediate the sensitive and flexible manipulation of intersectional neuronal populations in mice.

Sabatini PV, Wang J, Rupp AC, Affinati AH, Flak JN, Li C, Olson DP, Myers MG Jr
Elife. 2021 Mar 11;10. pii: 66835. doi: 10.7554/eLife.66835. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
166597pAAV-hSYN1-fDIO-tTA Encodes Flp-dependent tTA
166598pAAV-hSYN1-fDIO-rtTA Encodes Flp-dependent rtTA
166599pAAV-TRE-DIO-hM3Dq-mCherryEncodes Cre-dependent hM3Dq-mCherry under control of the TRE
166600pAAV-TRE-DIO-ChR2-TdTomatoEncodes Cre-dependent ChR2-TdTomato under control of the TRE
166601pAAV-TRE-DIO-Cas9Encodes Cre-dependent Cas9 under control of the TRE
166602pAAV-TRE-DIO-TVA-oG-mCherryEncodes Cre-dependent TVA-oG-mCherry under control of the TRE
166603pAAV-TRE-DIO-eGFP-2A-TetanusToxinEncodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE
166604pAAV-TRE-DIO-SwiChR-eYFPEncodes Cre-dependent SwiChR-eYFP under control of the TRE
166605pAAV-TRE-DIO-eGFP-L10aEncodes Cre-dependent eGFP-L10a under control of the TRE
166606pAAV-TRE-DIO-GCaMP6sEncodes Cre-dependent GCaMP6s under control of the TRE
166607pAAV-TRE-DIO-proCaspase-3-TEVpEncodes Cre-dependent procaspase 3 and TEVp under control of the TRE
166608pAAV-TRE-DIO-GFP-2A-SynmRubyEncodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE
166609pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE
166610pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)Encodes Cre-inactivated ChR2-TdTomato under control of the TRE
166611pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

Antibodies from Article