Directed evolution of biofuel-responsive biosensors for automated optimization of branched-chain alcohol biosynthesis.
Bahls MO, Platz L, Morgado G, Schmidt GW, Panke S
Metab Eng. 2021 Nov 9. pii: S1096-7176(21)00171-3. doi: 10.1016/j.ymben.2021.10.014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166502 | pCK01_PalkS_alkS | Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols. |
| 166503 | pAB_PalkB_sfgfp_KanR | Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols. |