Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy by promoting YAP phase separation.
Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, Zhang L, Li Y, Yang XP, Sun S
Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 10.1016/j.molcel.2021.01.010.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166439 | Pet22b-mEGFP | Bacterial expression of 6x His tagged mEGFP |
| 166440 | Pet22b-mEGFP-YAP-N-IDR | Bacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein |
| 166441 | Pet22b-mEGFP-YAP-C-IDR | Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein |
| 166442 | Pet22b-mCherry-TEAD4-IDR | Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein |
| 166443 | Pet22b-YAP | Bacterial expression of 6x His tagged YAP |
| 166444 | Pet22b-YAPL318E | Bacterial expression of 6x His tagged YAPL318E |
| 166445 | Pet22b-YAP4LE | Bacterial expression of 6x His tagged YAP4LE |
| 166446 | Lenti-Cry2-mCherry- YAP-N-IDR | Expresses fusion of CRY2PHR with mCherry and YAP-N-IDR |
| 166447 | Lenti-Cry2-mCherry- YAP-C-IDR | Expresses fusion of CRY2PHR with mCherry and YAP-C-IDR |
| 166448 | Lenti-Cry2-mCherry-coiled coil | Expresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain |
| 166449 | Lenti-Cry2-mCherry-TEAD4-IDR | Expresses fusion of CRY2PHR with mCherry and TEAD4-IDR |
| 166450 | pmCherry-TEAD4-YBD | Expresses fusion of mCherry and TEAD4-YAP binding domain |
| 166451 | pmCherry-TEAD4-IDR | Expresses fusion of mCherry and TEAD4-IDR |
| 166452 | pmCherry-N-EP300 | Expresses fusion of mCherry and EP300-N-IDR |
| 166454 | pmCherry-C-EP300 | Expresses fusion of mCherry and EP300-C-IDR |
| 166455 | Lenti-40XUAS-Luc | Luciferase reporter |
| 166457 | mEGFP-N1-YAP | Expresses fusion of mEGFP and YAP |
| 166458 | mEGFP-N1-YAPS127A | Expresses fusion of mEGFP and YAPS127A |
| 166459 | mEGFP-N1-YAPS127A-deltaCC | Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359) |
| 166460 | mEGFP-N1 YAPS127A-deltasIDR | Expresses fusion of mEGFP and YAPdeltasIDR (delta360-449) |
| 166461 | mEGFP-N1 YAPS127A-deltasTAD | Expresses fusion of mEGFP and YAPdeltasTAD (delta450-504) |
| 166462 | mEGFP-N1 YAPS127A- L308E | Expresses fusion of mEGFP and YAPS127A- L308E |
| 166464 | mEGFP-N1 YAPS127A- L325E | Expresses fusion of mEGFP and YAPS127A- L325E |
| 166465 | mEGFP-N1 YAPS127A- L318E | Expresses fusion of mEGFP and YAPS127A- L318E |
| 166466 | mEGFP-N1 YAPS127A- L308/311/325/318E (4LE) | Expresses fusion of mEGFP and YAPS127A- L308/311/325/318E (4LE) |
| 166467 | pHAGE-neo | Empty vector to express overexpression cDNA |
| 166468 | pHAGE-neo-YAP | Plasmid coding for YAP and neomycin resistance in a lentiviral backbone. |
| 166469 | pHAGE-neo-YAP-deltaCC | Plasmid coding for YAP-deltaCC (delta298-359) and neomycin resistance in a lentiviral backbone. |
| 166472 | pUC19-YAP-mEGFP | HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP |
| 166473 | mEGFP-N1-NF2 | Expresses fusion of mEGFP and NF2 |
| 166474 | mEGFP-N1-LATS2 | Expresses fusion of mEGFP and LATS2 |
| 166475 | mEGFP-N1-MST2 | Expresses fusion of mEGFP and MST2 |
| 166476 | mEGFP-N1-SAV1 | Expresses fusion of mEGFP and SAV1 |
| 166477 | mCherry-N1-MOB1 | Expresses fusion of mCherry and MOB1 |
| 166479 | PL-CRISPR.EFS.tRFP-gIfngr1-1 | Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1 |
| 166480 | PL-CRISPR.EFS.tRFP-gIfngr1-2 | Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1 |
| 166481 | Lenti-CRISPR-v2-gYap-1 | Expresses spCas9 and a gRNA targeting mouse Yap |
| 166482 | Lenti-CRISPR-V2-gYap-2 | Expresses spCas9 and a gRNA targeting mouse Yap |
| 166485 | PLKO.1-YAP shRNA2 | Human YAP RNAi |
| 166486 | PLKO.1-Yap shRNA | Mouse Yap RNAi |
| 166487 | PLKO.1-Cd155 shRNA1 | Mouse Cd155 RNAi |
| 166488 | PLKO.1-Cd155 shRNA2 | Mouse Cd155 RNAi |