CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations.
Fukushima T, Tanaka Y, Adachi K, Masuyama N, Tsuchiya A, Asada S, Ishiguro S, Mori H, Seki M, Yachie N, Goyama S, Kitamura T
Sci Rep. 2021 Dec 13;11(1):23889. doi: 10.1038/s41598-021-02986-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166230 | pLV-CS-154 | lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry |