Robust and flexible platform for directed evolution of yeast genetic switches.
Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A
Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165968 | pGK401red-rtetTAK8N, L131L | Expresses rTetTA (K8N, L131L) mutant |
| 165969 | pGK415-rtetTAK8N, L131L | Expresses rTetTA (K8N, L131L) mutant |
| 165970 | pGK415-phlTA2-1E | Expresses phlTA(2-1E) mutant |
| 165971 | pGK415-luxTA2-4F | Expresses luxTA(2-4F) mutant |
| 165975 | pATP416-pluxO5-ptetO7-crtYBI | Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA |
| 165976 | pATP416-ptetO7-pphlO6-crtYBI | Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA |
| 165977 | pATP416-pluxO5-pphlO6-crtYBI | Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA |
| 166027 | pATP403red-hENT1 | Expresses human equilibrative nucleoside transporter 1 (hENT1) |
| 166028 | pGK401red-hENT1 | Expresses human equilibrative nucleoside transporter 1 (hENT1) |
| 166029 | pRS405red-phlTA2-1E | Expresses phlTA(2-1E) mutant |
| 166030 | pRS403red-luxTA2-4F | Expresses luxTA(2-4F) mutant |