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Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase.

Li J, Mahata B, Escobar M, Goell J, Wang K, Khemka P, Hilton IB
Nat Commun. 2021 Feb 9;12(1):896. doi: 10.1038/s41467-021-21188-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
165601dCas9-MSK1Expresses Sp dCas9 fused to human MSK1
165602dCas9-dMSK1Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
165603dCas9-ddMSK1Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

Antibodies from Article