Drew Endy Lab: Open Enzyme Collection
Unpublished
Please visit the FreeGenes Project Open Enzyme Collection for more information.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165500 | pOpen-9N7polA | Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity |
| 165501 | pOpen-GBDpol | Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences. |
| 165502 | pOpen-9N7polA (CT) | 9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates |
| 165503 | pOpen-HSlig4 | Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction. |
| 165504 | pOpen-EcoRIR | Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1. |
| 165505 | pOpen-T7gene1 | Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage |
| 165506 | pOpen-Eco31I | Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI. |
| 165507 | pOpen-NotIR (GC) | Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1. |
| 165508 | pOpen-HindIIIR | Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1. |
| 165509 | pOpen-TEV protease | Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G |
| 165510 | pOpen-DNA Topoisomerase 1B Vaccinia Virus | Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence. |
| 165511 | pOpen-K12polLF | DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity. |
| 165512 | pOpen-THEAQpolA | DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing. |
| 165513 | pOpen-dbh | Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions. |
| 165514 | pOpen-9N7lig | 9 Degrees North DNA Ligase active at elevated temperatures (45 - 90 degrees C). |
| 165515 | pOpen-EcoRIM | Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI. |
| 165516 | pOpen-SP6rpol | DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes. |
| 165517 | pOpen-Eco31IA | Site-specific DNA methyltransferase 1; protects DNA from cleavage by Eco31I |
| 165518 | pOpen-NotIM | Site-specific DNA methyltransferase; protects DNA from cleavage by NotI |
| 165520 | pOpen-T4 PNK | The most commonly used polynucleotide kinase. |
| 165521 | pOpen-lambda red operon | Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA. |
| 165522 | pOpen-K12polLF (exo-) | DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments. |
| 165523 | pOpen-Tlipol (exo-) | DNA polymerase; lacks 3' to 5' exonuclease activity. |
| 165524 | pOpen-T4gene43 | Polymerase that replicates viral genomic DNA. |
| 165525 | pOpen-Pfulig | DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. |
| 165526 | pOpen-PstIR | Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5. |
| 165527 | pOpen-Eco31IB | Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I |
| 165528 | pOpen-SfiIR (cDNA) | Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4. |
| 165529 | pOpen-DpnIR | Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA) |
| 165530 | pOpen-T4-BGT | T4 Phage beta-glucosyltransferasetransfers the glucose moiety of UDP-Glc to the 5-hmC residues in double-stranded DNA. |
| 165531 | pOpen-UDG | Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil. |
| 165532 | pOpen-ECOpolA | DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity. |
| 165533 | pOpen-TthpolA | DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C). |
| 165534 | pOpen-T7gene5 | DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease. |
| 165535 | pOpen-THEAQlig | Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction |
| 165536 | pOpen-PstIM | Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI. |
| 165537 | pOpen-SapIR | Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1. |
| 165538 | pOpen-SfiIM | Site-specific DNA methyltransferase; protects DNA from cleavage by SfiI. |
| 165539 | pOpen-Bst HF | High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences. |
| 165540 | pOpen-T7EI | DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform). |
| 165541 | pOpen-Fpg | Formamidopyrimidine DNA Glycosylase; Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities |
| 165542 | pOpen-KTHEAQpol | Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase. |
| 165543 | pOpen-Bstpol | Full length DNA polymerase from Bacillus stearothermophilus. |
| 165544 | pOpen-T5gene122 | Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes. |
| 165546 | pOpen-MMLV_RT (mut H) | Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. |
| 165547 | pOpen-SapIM1 | Site-specific DNA methyltransferase 1; protects DNA from cleavage by SapI |
| 165548 | pOpen-EcoRV | Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1. |
| 165549 | pOpen-ETSSB | ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure |
| 165550 | pOpen-T4 PGD | T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. |
| 165551 | pOpen-Endonuclease IV E. coli | DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. |
| 165553 | pOpen-BstpolLF | Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement. |
| 165554 | pOpen-T4gene30 | Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair. |
| 165555 | pOpen-ALPI | CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used |
| 165556 | pOpen-MMLV_RT (lack H) | Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher. |
| 165557 | pOpen-SapIM2 | Site-specific DNA methyltransferase 2; protects DNA from cleavage by SapI |
| 165558 | pOpen-EcoRVM | Site-specific DNA methyltransferase; protects DNA from cleavage by EcoRV. |
| 165559 | pOpen-T5gene12/13 | Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction. |
| 165560 | pOpen-T4 g32 | A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples. |
| 165561 | pOpen-Endonuclease VIII E. coli | Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini. |
| 165562 | pOpen-Pwopol | DNA polymerase; additionally exhibits 3' to 5' exonuclease activity. |
| 165563 | pOpen-BsupolLF | Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis. |
| 165564 | pOpen-ECOligA | Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided. |
| 165565 | pOpen-RNAse A | Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA. |
| 165566 | pOpen-TthRT | DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2 |
| 165567 | pOpen-XbaIR | Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1. |
| 165568 | pOpen-NcoIR | Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1. |
| 165569 | pOpen-Tthlig | Taq DNA Ligase |
| 165570 | pOpen-P45 | Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo. |
| 165571 | pOpen-Exonuclease III E. coli | Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA. |
| 165572 | pOpen-GBDpol (exo) | Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB |
| 165573 | pOpen-phi29pol | DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity. |
| 165574 | pOpen-HSlig3 | Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992)) |
| 165575 | pOpen-RNAse H | Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication. |
| 165576 | pOpen-T3gene1 | DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. |
| 165577 | pOpen-XbaIM | Site-specific DNA methyltransferase; protects DNA from cleavage by XbaI |
| 165578 | pOpen-NcoIM | Site-specific DNA methyltransferase; protects DNA from cleavage by NcoI |
| 165579 | pOpen-dromedNK | Used for dNTP synthesis |
| 165580 | pOpen-BovDNTT | Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR |