PACmn for improved optogenetic control of intracellular cAMP.
Yang S, Constantin OM, Sachidanandan D, Hofmann H, Kunz TC, Kozjak-Pavlovic V, Oertner TG, Nagel G, Kittel RJ, Gee CE, Gao S
BMC Biol. 2021 Oct 18;19(1):227. doi: 10.1186/s12915-021-01151-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165488 | pGEM-HE-bPAC(R278A)-Myc-eYFP | Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity |
| 165489 | pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y) | Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity |
| 165490 | pGEM-HE-Glyco-Venus-bPAC(S27A) | Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity |
| 165491 | pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y) | Neuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity . |
| 165492 | pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y) | Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity |
| 165493 | pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y) | Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity |
| 171117 | pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y) | Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity |