Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival.
Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH, Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 10.1083/jcb.202005051.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166946 | UbC-Halo-SFPQ | Lentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoter |
| 166947 | UbC-Halo-SFPQY527A | Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter |
| 166948 | pLV-EF1a-HaloSFPQ | Lentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a promoter |
| 166949 | pLV-EF1a-HaloSFPQY527A | Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter |
| 166950 | pLV-EF1a-GFP-SFPQ-IRES-mCherry | Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter |
| 166951 | pLV-EF1a-GFP-SFPQY527A-IRES-mCherry | Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter |
| 166952 | pLV-EF1a-KIF5A-HA-IRES-Puro | Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter |
| 166953 | pLV-EF1a-KIF5AR280H-HA-IRES-Puro | Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter |
| 166954 | FLAG-KIF5A | Expresses FLAG-tagged KIF5A protein in pcDNA3.1 |
| 166955 | FLAG-KIF5A delta Tail | Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1 |
| 166956 | FLAG-KIF5A tail only | Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1 |
| 166957 | FLAG-KIF5AR280H | Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1 |
| 166958 | HA-KIF5A | Expresses HA-tagged KIF5A protein in pcDNA3.1 |
| 166959 | HA-SFPQ | Expresses HA-tagged SFPQ protein in pcDNA3.1 |
| 166960 | FLAG-SFPQ | Expresses FLAG-tagged SFPQ protein in pcDNA3.1 |
| 166961 | FLAG-SFPQY527A | Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1 |
| 166962 | KLC1-Myc | Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1 |
| 166963 | KLC1N343S-Myc | Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1 |
| 166964 | Myc-KLC1 | Expresses Myc-tagged KLC1 protein in pcDNA3.1 |
| 166965 | Myc-KLC1 delta Tail | Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1 |