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An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters.

Hamar J, Kultz D
Sci Rep. 2021 Apr 12;11(1):7854. doi: 10.1038/s41598-021-87068-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
165484OmEF1aCas9P2ApuroSBStable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
165485TU6m-gRNAscaffHygroRS. pyogenes Cas9 guide RNA expression in tilapia cells

Antibodies from Article