An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters.
Hamar J, Kultz D
Sci Rep. 2021 Apr 12;11(1):7854. doi: 10.1038/s41598-021-87068-3.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
165484 | OmEF1aCas9P2ApuroSB | Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells |
165485 | TU6m-gRNAscaffHygroR | S. pyogenes Cas9 guide RNA expression in tilapia cells |