Photoactivatable CaMKII induces synaptic plasticity in single synapses.
Shibata ACE, Ueda HH, Eto K, Onda M, Sato A, Ohba T, Nabekura J, Murakoshi H
Nat Commun. 2021 Feb 2;12(1):751. doi: 10.1038/s41467-021-21025-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165429 | pAAV-CaMP0.4-FHS-paCaMKII-WPRE3 | Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity. |
| 165430 | pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3 | Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control. |
| 165431 | CMV-tdTomato-P2A-paCaMKII | Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity. |
| 165432 | CMV-tdTomato-P2A-paCaMKII(T286A) | Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity. |
| 165433 | CMV-tdTomato-P2A-paCaMKII(K42M) | Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control. |
| 165434 | CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42 | Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging. |
| 165435 | CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA | Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging. |
| 165436 | CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42 | Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging. |
| 165437 | CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA | Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging. |
| 165438 | CMV-mEGFP(A206K)-paCaMKII | Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity. |
| 165439 | CMV-mEGFP(A206K)-paCaMKII(T286A) | Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity. |
| 165440 | CMV-mEGFP(A206K)-paCaMKII(K42M) | Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control. |
| 167475 | CMV-mEGFP(A206K)-paCaMKII-ShadowG | Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII. |
| 167476 | CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG | Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII. |
| 167477 | CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG | Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII. |
| 167478 | CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG | Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII. |
| 167479 | CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG | Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII. |