CRISPR-Cas9 Based Genome Editing in Wheat.
Smedley MA, Hayta S, Clarke M, Harwood WA
Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165422 | pGoldenGreenGate-M (pGGG-M) | A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000). |
| 165423 | Level 1 P1 OsActinP:Hygint:NosT | GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette |
| 165424 | Level 1 P2 OsUbiP:Cas9:NosT | GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette |
| 165599 | Level 1 P3 TaU6 guide acceptor | GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid |
| 165600 | Level 1 P4 TaU6 guide acceptor | GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid |