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Overexpression of Key Sterol Pathway Enzymes in Two Model Marine Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum.

Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, Ralph PJ
Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 10.3390/ph13120481. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
139961pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAGOver-expression of heterologus squalene epoxidase from Nannochloropsis oceanica
139963pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAGOver-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase
139964pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAGOver-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

Antibodies from Article