Cas9 deactivation with photocleavable guide RNAs.

Zou RS, Liu Y, Wu B, Ha T
Mol Cell. 2021 Apr 1;81(7):1553-1565.e8. doi: 10.1016/j.molcel.2021.02.007. Epub 2021 Mar 3. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
165157	pET42b-AncBE4max	Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression
165158	pLPC-mCh-ACTB-P2A-EGFP	Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells