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A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types.

Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs D, Barelli H, Antonny B
Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub 2021 May 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
165108Nsp3 -EGFPmammalian expression and localization
165109Nsp4-EGFPmammalian expression and localization
165111Nsp7-EGFPmammalian expression and localization
165112EGFP-Nsp7mammalian expression and localization
165113Nsp8-EGFPmammalian expression and localization
165114EGFP-Nsp8mammalian expression and localization
165115Nsp10-EGFPmammalian expression and localization
165116Nsp12-EGFPmammalian expression and localization
165117EGFP-Nsp12mammalian expression and localization
165119Nsp14-EGFPmammalian expression and localization
165120EGFP-Nsp15mammalian expression and localization
165121ORF3a-EGFPmammalian expression and localization
165122EGFP-ORF9bmammalian expression and localization
165123EGFP-COV Emammalian expression and localization
165124EGFP-COV Mmammalian expression and localization
165125Nsp14D-EGFPmammalian expression and localization
165126Nsp4 deltaC-EGFPmammalian expression and localization
165127Nsp13 deltaN1-EGFPmammalian expression and localization
165128Nsp13 deltaN2-EGFP mammalian expression and localization
165129EGFP-Orf9b L52D mammalian expression and localization
165130Nsp13 K289A-EGFPmammalian expression and localization
165131Nsp3 -mCherrymammalian expression and localization
165132Nsp4-mCherrymammalian expression and localization
165133Nsp6-mCherrymammalian expression and localization
165134Nsp7-mCherrymammalian expression and localization
165135Nsp10-mCherrymammalian expression and localization
165136Nsp13-mCherrymammalian expression and localization
165137Nsp14-mCherrymammalian expression and localization
165138Orf3a-mCherrymammalian expression and localization
165139Nsp14D-mCherrymammalian expression and localization

Antibodies from Article