Screening for functional circular RNAs using the CRISPR-Cas13 system.
Li S, Li X, Xue W, Zhang L, Yang LZ, Cao SM, Lei YN, Liu CX, Guo SK, Shan L, Wu M, Tao X, Zhang JL, Gao X, Zhang J, Wei J, Li J, Yang L, Chen LL
Nat Methods. 2021 Jan;18(1):51-59. doi: 10.1038/s41592-020-01011-4. Epub 2020 Dec 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165070 | p23-NES-LwaCas13a-msfGFP-NES-Flag | overexpression of LwaCas13a in human cells |
| 165071 | p23-NES-PspCas13b-msfGFP-NES-Flag | overexpression of PspCas13b in human cells |
| 165072 | p23-NES-PguCas13b-msfGFP-NES-Flag | overexpression of PguCas13b in human cells |
| 165073 | p23-NES-RanCas13b-msfGFP-NES-Flag | overexpression of RanCas13b in human cells |
| 165074 | p23-NES-EsCas13d-msfGFP-NES-Flag | overexpression of EsCas13d in human cells |
| 165075 | p23-NES-AdmCas13d-msfGFP-NES-Flag | overexpression of AdmCas13d in human cells |
| 165076 | p23-NES-RfxCas13d-msfGFP-NES-Flag | overexpression of RfxCas13d in human cells |
| 165077 | p23-NLS-RfxCas13d-mcherry-NLS-Flag | overexpression of RfxCas13d in human cells |
| 165078 | RfxCas13d-backbone | Expression of RfxCas13d sgRNAs from a U6 promoter |