Amino Acids Enhance Polyubiquitination of Rheb and Its Binding to mTORC1 by Blocking Lysosomal ATXN3 Deubiquitinase Activity.
Yao Y, Hong S, Ikeda T, Mori H, MacDougald OA, Nada S, Okada M, Inoki K
Mol Cell. 2020 Nov 5;80(3):437-451.e6. doi: 10.1016/j.molcel.2020.10.004.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165022 | pLJM1-Flag-Rheb-4Rs | expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines) |
| 165023 | pLJM1-Flag-Rheb-12Rs | expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines). |
| 165031 | pLJM1-Flag-Rheb | expression of Flag tagged Rheb protein in mammalian cells |