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Structural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex.

Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, Lim CY, Samelson AJ, Hurley JH, Zoncu R
Science. 2019 Nov 22;366(6468):971-977. doi: 10.1126/science.aax0364. Epub 2019 Oct 31. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164978pLJM1 FLCN (WT) FLAG GFPLentiviral vector for FLCN expression in mammalian cells
164979pLJM1 FLCN (F118D) FLAG GFPLentiviral vector for FLCN expression in mammalian cells
164980pLJM1 FLCN (R164A) FLAG GFPLentiviral vector for FLCN expression in mammalian cells
164981pCAG-TWIN-STREP-FLAG-FLCNFLCN expressing vector for mammalian cells
164982pCAG-GST FNIP2FNIP2 expressing vector for mammalian cells
164983pCAG GST DEPDC5DEPDC5 expressing vector for mammalian cells
164984pCAG-TWIN-STREP-FLAG-NPRL3NPRL3 expressing vector for mammalian cells
164985pCAG-TWIN-STREP-FLAG-NPRL2NPRL2 expressing vector for mammalian cells

Antibodies from Article