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Potassium Channel-Associated Bioelectricity of the Dermomyotome Determines Fin Patterning in Zebrafish.

Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, Mittal SK, Zhang G
Genetics. 2020 Aug;215(4):1067-1084. doi: 10.1534/genetics.120.303390. Epub 2020 Jun 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164940pDestTol2-actinb-kcnj13-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus
164941pDestTol2-actinb-kcnj13-IRES-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP
164942pDestTol2-actinb-kcnj13-T131A-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R
164943pDestTol2-actinb-kcnj13-Q153H-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I
164944pDestTol2-actinb-kcnj13-M135R-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135R
164945pDestTol2-actinb-Hs.KCNJ13-IRES-EGFPTol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP
164946pDestTol2-actinb-kcnj1b-IRES-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP
164947pDestTol2-actinb-kcnj10a-IRES-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP
164949pDestTol2-actinb-kcnk9-IRES-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP
164950pDestTol2-pax3a-kcnj13-IRES-EGFPTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP
164965pDestTol2-actinb-kcna1a-IRES-EGFPTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP
164966p5E-pax3a-5.4kzebrafish pax3a -5.4k promoter

Antibodies from Article