Oncogenesis driven by the Ras/Raf pathway requires the SUMO E2 ligase Ubc9.
Yu B, Swatkoski S, Holly A, Lee LC, Giroux V, Lee CS, Hsu D, Smith JL, Yuen G, Yue J, Ann DK, Simpson RM, Creighton CJ, Figg WD, Gucek M, Luo J
Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):E1724-33. doi: 10.1073/pnas.1415569112. Epub 2015 Mar 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164936 | MSCVhyg-HA-FLAG_Ubc9_WT | Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA |
| 164937 | MSCVhyg-HA-FLAG_Ubc9_C93A | Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA |
| 164938 | MSCVpuro-6xHis_SUMO2 | Expresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA |
| 164939 | pHAGE-CMV-6xHis-SUMO1 | Expresses 6xHis tagged human Small Ubiquitin Like Modifier 1 (SUMO1) wild type cDNA |
| 168984 | pInducer10-SAE1_sh1 | Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells |
| 168985 | pInducer10-SAE1_sh3 | Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells |
| 168986 | pInducer10-UBC9-sh1 | Expresses RFP cDNA and UBC9 shRNA 1 in mammalian cells |
| 168987 | pInducer10-UBC9-sh3 | Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells |
| 168988 | pInducer10-UBC9-sh4 | Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells |