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A high-throughput assay for small molecule destabilizers of the KRAS oncoprotein.

Carver J, Dexheimer TS, Hsu D, Weng MT, Smith JL, Guha R, Jadhav A, Simeonov A, Luo J
PLoS One. 2014 Aug 5;9(8):e103836. doi: 10.1371/journal.pone.0103836. eCollection 2014. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164925pInducer10b-EGFP-KRAS G12VExpresses EGFP cDNA and KRAS G12V cDNA in mammalian cells
164935pInducer10b-EGFP-KRAS-WTExpresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

Antibodies from Article