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REPAIRx, a specific yet highly efficient programmable A > I RNA base editor.

Liu Y, Mao S, Huang S, Li Y, Chen Y, Di M, Huang X, Lv J, Wang X, Ge J, Shen S, Zhang X, Liu D, Huang X, Chi T
EMBO J. 2020 Nov 16;39(22):e104748. doi: 10.15252/embj.2020104748. Epub 2020 Oct 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164877TC1405BFP-preSTOP-GFP reporter
164878TC1078-3030bp(mismatch17) cas13d gRNA for 1405 editing
164879TC1092CMV-dcas13d-NLS-ADARdd- mCherry
164881TC1464CMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1
164883TC1316CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3

Antibodies from Article