Skip to main content
Addgene

Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems.

Wandera KG, Beisel CL
Methods Mol Biol. 2022;2404:135-153. doi: 10.1007/978-1-0716-1851-6_7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164857pLsCas13a-gRNA-NTConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.
164858pBzCas13b-gRNA-1Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.
164859pBzCas13b-gRNA-2Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.
164860pBzCas13b-gRNA-3Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.
164861pBzCas13b-gRNA-NTConstitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.
164862pBzCas13bInducible expression of BzCas13b nuclease in bacteria.
164863pAcrVIA4Inducible expression of AcrVIA4 in bacteria.
164864pAcrVIA7Inducible expression of AcrVIA7 in bacteria.
164865pLsCas13a-gRNA-1Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.
164866pLsCas13a-gRNA-2Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.
164867pLsCas13a-gRNA-3Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Antibodies from Article