MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits.
Ma L, Ruan J, Song J, Wen L, Yang D, Zhao J, Xia X, Chen YE, Zhang J, Xu J
Nat Commun. 2020 Nov 27;11(1):6082. doi: 10.1038/s41467-020-19842-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162758 | pmiCas9 | express miCas9 |
| 164832 | pmiHiFiCas9 | miHiFiCas9 expressing plasmid |
| 164833 | pmiCas9-msa | miCas9-msa fusion protein expressing plasmid |