T cell circuits that sense antigen density with an ultrasensitive threshold.
Hernandez-Lopez RA, Yu W, Cabral KA, Creasey OA, Lopez Pazmino MDP, Tonai Y, De Guzman A, Makela A, Saksela K, Gartner ZJ, Lim WA
Science. 2021 Mar 12;371(6534):1166-1171. doi: 10.1126/science.abc1855. Epub 2021 Feb 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164822 | pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133 | Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch |
| 164823 | pHR_SFFv_Her2-BFP_RHL027 | Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP |
| 164824 | pHR_SFFv_4D5-Low-CAR_RHL001 | Lentiviral vector for constitutive expression of AntiHER2 4D5-Low CAR |
| 164825 | pHR_SFFv_4D5-High-CAR_RHL002 | Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR |
| 164826 | pHR_SFFv_4D5-WT-Highest-CAR_RHL004 | Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR |
| 164827 | pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183 | Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch |
| 164828 | pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144 | Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch |