CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes.
Muller R, Meacham ZA, Ferguson L, Ingolia NT
Science. 2020 Dec 11;370(6522). pii: 370/6522/eabb9662. doi: 10.1126/science.abb9662.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164912 | pNTI732 pRPR1(TetO)-HTS1-sgRNA | For yeast genomic integration of sgRNA against HTS1 |
| 164913 | pNTI733 pRPR1(TetO)-RPC31-sgRNA | For yeast genomic integration of sgRNA against RPC31 |
| 164915 | pNTI743 dual-barcoded gRNA parent vector | CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries |
| 164916 | pNTI751 pPGK1-GCN4(UTR)-ZEM(TF) | Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR |
| 164917 | pNTI757 P(PGK1)-citrine P(HIS4)-mCherry | Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer |
| 164918 | pNTI763 p(Z)-mCherry + UBC9 sgRNA | CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA |