Microbial single-strand annealing proteins enable CRISPR gene-editing tools with improved knock-in efficiencies and reduced off-target effects.
Wang C, Cheng JKW, Zhang Q, Hughes NW, Xia Q, Winslow MM, Cong L
Nucleic Acids Res. 2021 Apr 6;49(6):e36. doi: 10.1093/nar/gkaa1264.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164802 | pREDIT_Cas9-MS2-BB_BbsI | REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP |
| 164803 | pREDIT_MCP-RecT | mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS |
| 164804 | pREDIT_Cas9n-MS2-BB_BbsI | REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP |
| 164805 | pCBH-Cas9-Suntag-BB_BbsI | REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP |