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A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase.

Toth AD, Garger D, Prokop S, Soltesz-Katona E, Varnai P, Balla A, Turu G, Hunyady L
J Biol Chem. 2021 Feb 2:100366. doi: 10.1016/j.jbc.2021.100366. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164783GLuc-PMGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
164784NanoLuc-PMNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
164785GLuc-PM-VenusGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
164786NanoLuc-PM-VenusNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

Antibodies from Article