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Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor.

Gligorovski V, Sadeghi A, Rahi SJ
Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164702pVG10yEVenus under the control of GALL promoter
164703pVG45yEVenus under the control of CUP1 promoter
164704pVG46yEVenus under the control of PHO5 promoter
164705pVG47yEVenus under the control of tetOpr
164706pVG48rtTA under the control of PGK1 promoter
164707pVG49yEVenus under the control of GAL1 promoter
164708pVG50yEVenus under the control of tetOpr with ADH1t upstream of the reporter
164709pVG88yEVenus under the control of ARG3 promoter
164710pCL10yEVenus under the control of MET3 promoter
164849pVG97Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct
211765pVG122Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

Antibodies from Article