Günter Schneider Lab: sgRNA MS2 (zeo_backbone) target c-MYC

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
164636	sgRNA(MS2)_zeo insert c-MYC	sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
164637	lentiGuide-Puro PRMT5-CRISPRi	lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2