A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo.
Dong A, He K, Dudok B, Farrell JS, Guan W, Liput DJ, Puhl HL, Cai R, Wang H, Duan J, Albarran E, Ding J, Lovinger DM, Li B, Soltesz I, Li Y
Nat Biotechnol. 2021 Nov 11. pii: 10.1038/s41587-021-01074-4. doi: 10.1038/s41587-021-01074-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164604 | pAAV-hSyn-GRAB_eCB2.0 | Express the endocannabinoid sensor GRAB_eCB2.0 in neurons |
| 164605 | pAAV-hSyn-GRAB_eCBmut | Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons |
| 164606 | pAAV-hSyn-DIO-GRAB_eCB2.0 | Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons |
| 164607 | pAAV-hSyn-DIO-GRAB_eCB2.0mut | Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons |
| 164608 | pAAV-CaMKII-GRAB_eCB2.0 | Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons |
| 164609 | pAAV-GfaABC1D-GRAB_eCB2.0 | Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes |
| 164610 | pAAV-EFS-fDIO-GRAB_eCB2.0 | Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells |
| 164611 | pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX | Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells |
| 164612 | pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX | Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells |