Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.
Desai PB, Stuck MW, Lv B, Pazour GJ
J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 10.1083/jcb.201912104.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164526 | MS03 | Expresses Sonic Hedgehog |
| 164527 | GP734 | Expresses Flag-Ub |
| 164528 | PD19 | Expresses Flag-Ub noK |
| 164529 | PD14 | Expresses HA-Ub |
| 164530 | PD13 | Expresses HA-Ub noK |
| 164531 | PD22 | Expresses Smo-FLAG |
| 164532 | GP736 | Express Smo-FLAG-Ub |
| 164533 | GP777 | Expresses Smo noK-FLAG |
| 164534 | GP806 | Expresses Smo noK-FLAG-Ub |
| 164535 | GP807 | Expresses Smo noK-FLAG-Ub noK |
| 164536 | PD23 | Expresses SmoM2-FLAG |
| 164537 | GP735 | Expresses SmoM2-FLAG-Ub |
| 164538 | GP938 | Expresses SmoPi-FLAG |
| 164539 | GP940 | Expresses SmoPi-FLAG-Ub |
| 164540 | GP757 | Expresses Sstr3-FLAG |
| 164541 | GP758 | Expresses Sstr3-FLAG-Ub |
| 164542 | PD29 | Expresses Drd1-FLAG |
| 164543 | PD30 | Expresses Drd1-Ub |
| 164544 | GP799 | Expresses Smo-FLAG |
| 164545 | GP805 | Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R |
| 164546 | GP802 | Expresses Smo-FLAG K348, 360R |
| 164547 | GP803 | Expresses Smo-FLAG K434, 444, 448R |
| 164548 | GP804 | Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R |
| 164549 | GP824 | Expresses Smo-FLAG K434, 444R |
| 164550 | GP825 | Expresses Smo-FLAG K434, 448R |
| 164551 | GP826 | Expresses Smo-FLAG K444, 448R |
| 164552 | GP821 | Expresses Smo-FLAG K434R |
| 164553 | GP822 | Expresses Smo-FLAG K444R |
| 164554 | GP823 | Expresses Smo-FLAG K448R |