Structure of bacterial phospholipid transporter MlaFEDB with substrate bound.
Coudray N, Isom GL, MacRae MR, Saiduddin MN, Bhabha G, Ekiert DC
Elife. 2020 Nov 25;9. pii: 62518. doi: 10.7554/eLife.62518.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162132 | pBEL2139 | Expression and Complementation plasmid for MlaD TM swap with LptC TM |
| 162133 | pBEL2138 | Expression and Complementation plasmid for MlaD TM swap with LetB TM |
| 162134 | pBEL1198 | Expression and complementation plasmid for MlaFEDCB, with N-terminal his tag on MlaE |
| 162135 | pBEL2093 | Expression and Complementation plasmid for MlaE IF1 1-15 aa deletion |
| 162136 | pBEL2132 | Expression and Complementation plasmid for MlaE IF1 1-25 aa deletion |
| 162137 | pBEL2092 | Expression and Complementation plasmid for MlaE IF1 1-30 aa deletion |
| 162138 | pBEL2133 | Expression and Complementation plasmid for MlaE IF1 1-39 aa deletion |
| 162139 | pBEL2099 | Complementation plasmid for MlaE Y81A |
| 162140 | pBEL2100 | Complementation plasmid for MlaE Y81W |
| 162141 | pBEL2098 | Complementation plasmid for MlaE R97A |
| 162142 | pBEL2094 | Complementation plasmid for MlaE E98A |
| 162143 | pBEL2095 | Complementation plasmid for MlaE K205A |
| 162144 | pBEL2097 | omplementation plasmid for MlaE D250A |
| 162145 | pBEL2057 | Expression plasmid for MlaE Tyr81Bpa mutant for in vivo crosslinking |
| 162146 | pBEL2060 | Expression plasmid for MlaE Val77Bpa mutant for in vivo crosslinking |
| 162147 | pBEL2061 | Expression plasmid for MlaE Leu78Bpa mutant for in vivo crosslinking |
| 162148 | pBEL2062 | Expression plasmid for MlaE Leu70Bpa mutant for in vivo crosslinking |
| 162149 | pBEL2063 | Expression plasmid for MlaE Leu99Bpa mutant for in vivo crosslinking |
| 162150 | pBEL2065 | Expression plasmid for MlaE Trp149Bpa mutant for in vivo crosslinking |
| 162151 | pBEL2066 | Expression plasmid for MlaE Phe209Bpa mutant for in vivo crosslinking |
| 164596 | pBEL2290 | Complementation plasmid for MlaD (G11F) |
| 164597 | pBEL2291 | Complementation plasmid for MlaD (I12F) |
| 164598 | pBEL2292 | Complementation plasmid for MlaD (G11F, I12F) |
| 164599 | pBEL2294 | Complementation plasmid for MlaD (F13A) |
| 164600 | pBEL2295 | Complementation plasmid for MlaD (A17F) |
| 164601 | pBEL2296 | Complementation plasmid for MlaD (A20F) |
| 164602 | pBEL2297 | Complementation plasmid for MlaD (V24F) |
| 164603 | pBEL2298 | Expression and Complementation plasmid for MlaD (A17F, A20F, V24F) |