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COVID-19-neutralizing antibodies predict disease severity and survival.

Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG, Iafrate AJ, Balazs AB
Cell. 2021 Jan 21;184(2):476-488.e11. doi: 10.1016/j.cell.2020.12.015. Epub 2020 Dec 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164432pHAGE-CMV-Luc2-IRES-ZsGreen-WLentiviral vector backbone encoding firefly luciferase and ZsGreen
164433pHDM-SARS-CoV-2Encodes SARS-CoV-2 Spike Protein for pseudovirus production
164434pHDM-SARS-CoV-2 Δ18Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
164435pTwist-SARS-CoV-2Encodes SARS-CoV-2 Spike Protein for pseudovirus production
164436pTwist-SARS-CoV-2 Δ18Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
164437pTwist-SARS-CoV-2 Δ18 D614GEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production
164438pTwist-WIV1-CoVEncodes WIV1-CoV Spike Protein for pseudovirus production
164439pTwist-WIV1-CoV Δ18Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
164440pHDM-VSV-GEncodes VSV-G protein for pseudovirus production
164441pHDM-Hgpm2Encodes codon-optimized HIV gagpol for pseudovirus production
164442pHDM-Tat1bEncodes HIV-1 Tat accessory protein for pseudovirus production
164443pRC-CMV-Rev1bEncodes HIV-1 Rev accessory protein for pseudovirus production

Antibodies from Article