Prime Editing Guide RNA Design Automation Using PINE-CONE.
Standage-Beier K, Tekel SJ, Brafman DA, Wang X
ACS Synth Biol. 2021 Feb 19;10(2):422-427. doi: 10.1021/acssynbio.0c00445. Epub 2021 Jan 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164423 | pHSG1C3 | single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs. |