A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells.
Brookhouser N, Nguyen T, Tekel SJ, Standage-Beier K, Wang X, Brafman DA
BMC Biol. 2020 Dec 14;18(1):193. doi: 10.1186/s12915-020-00929-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164411 | pEF-XMAS-1xStop | Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon. |
| 164412 | pEF-XMAS-2xStop | Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons. |
| 164413 | pDT-sgRNA-XMAS-1x | Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites. |
| 164414 | pDT-sgRNA-XMAS-2x | Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites. |
| 164415 | pEF-ABEmax | EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector. |