Efficient engineering of human and mouse primary cells using peptide-assisted genome editing.
Zhang Z, Baxter AE, Ren D, Qin K, Chen Z, Collins SM, Huang H, Komar CA, Bailer PF, Parker JB, Blobel GA, Kohli RM, Wherry EJ, Berger SL, Shi J
Nat Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01756-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199604 | Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP) | Expression of cell permeable spCas9 in E. Coli. |
| 199605 | opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP) | Expression of cell permeable opAsCas12a in E. Coli. |