Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant.
Adalsteinsson BT, Kristjansdottir T, Merre W, Helleux A, Dusaucy J, Tourigny M, Fridjonsson O, Hreggvidsson GO
Sci Rep. 2021 May 5;11(1):9586. doi: 10.1038/s41598-021-89029-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164264 | pTTCC | Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus |