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Improved dynamic range of a rhamnose-inducible promoter for gene expression in Burkholderia.

Hogan AM, Jeffers KR, Palacios A, Cardona ST
Appl Environ Microbiol. 2021 Jun 30:AEM0064721. doi: 10.1128/AEM.00647-21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164226pSCrhaB2plusBroad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.
164227pSC201plusIntegrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression

Antibodies from Article