ER-directed TREX1 limits cGAS activation at micronuclei.

Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 10.1016/j.molcel.2020.12.037. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
164225	pLENTI-CMV-GFP-TREX1 D18N BLAST	lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)
164228	pLENTI-CMV-GFP-TREX1 BLAST	lentiviral plasmid for GFP-hTREX1
164229	pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST	lentiviral plasmid for GFP-hTREX1 delta C (aa1-235)
164231	pQCXIP-GFP-RPA70	retroviral plasmid for GFP-RPA70
164232	pQCXIZ-turq-3xFLAG-TREX1	retroviral plasmid for turq-3xFLAG-TREX1
164233	pQCXIZ-turq-3xFLAG-TREX1 D18N	retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)
164234	pLENTI CMV GFP TREX1 R128A R174A BLAST	lentiviral plasmid for GFP-TREX1 R128A R174A
164235	pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST	lentiviral plasmid for GFP-TREX1 delta N (aa236-314)
164236	pLENTI CMV GFP-TREX1 V235fs BLAST	lentiviral plasmid for GFP-TREX1 V235fs
164237	pLENTI CMV GFP-TREX1 D272fs BLAST	lentiviral plasmid for GFP-TREX1 D272fs
164238	pLENTI CMV GFP-TREX1 L287fs BLAST	lentiviral plasmid for GFP-TREX1 L287fs
164239	pLENTI CMV GFP-TREX1 P290L BLAST	lentiviral plasmid for GFP-TREX1 P290L
164240	pLENTI CMV GFP-TREX1 Y305C BLAST	lentiviral plasmid for GFP-TREX1 Y305C
164241	pLENTI CMV GFP-TREX1 G306A BLAST	lentiviral plasmid for GFP-TREX1 G306A
164242	pLENTI CMV GFP-TREX1 309* BLAST	lentiviral plasmid for GFP-TREX1 309*
164243	pQCXIZ 3xFLAG-TREX1	retroviral plasmid for 3xFLAG-TREX1
164244	pQCXIB-H2B-mCherry	retroviral plasmid for H2B-mCherry
164245	pQCXIP GFP-TREX1	retroviral plasmid for GFP-TREX1
164248	pQCXIP-GFP-BAF	retroviral plasmid for GFP-BAF
164249	pQCXIP-GFP-LmnB1	retroviral plasmid for GFP-LmnB1
164250	pU6-TREX1 g1 Cas9-T2A-mCherry	Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells
164251	pU6-TREX1 g2 Cas9-T2A-mCherry	Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells
164252	pU6-TREX1 g3 Cas9-T2A-mCherry	Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells
164253	pU6-cGAS g2 Cas9-T2A-mCherry	Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells
164254	pU6-cGAS g3 Cas9-T2A-mCherry	Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells
164255	pHP138 Myc-TRF2-deltaB deltaM	for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid
164256	pU6-p53 gRNA-Cas9-T2A-mCherry	Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells