Freeze-Frame Imaging of Dendritic Calcium Signals With TubuTag.
Perez-Alvarez A, Huhn F, Durst CD, Franzelin A, Lamothe-Molina PJ, Oertner TG
Front Mol Neurosci. 2021 Mar 4;14:635820. doi: 10.3389/fnmol.2021.635820. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164184 | pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag) | Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018) |
| 164193 | pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag) | Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM) |