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Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan elaboration.

Yang Q, Hughes TA, Kelkar A, Yu X, Cheng K, Park S, Huang WC, Lovell JF, Neelamegham S
Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164219ACE2Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker
164220S1-FcExpresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter
164221RBD-FcExpresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter
164222ACE2-FcExpresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

Antibodies from Article