Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins.
Tanida I, Furuta Y, Yamaguchi J, Kakuta S, Oliva Trejo JA, Uchiyama Y
Sci Rep. 2020 Dec 14;10(1):21871. doi: 10.1038/s41598-020-78879-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164161 | pCAG-mWasabi-G | Expresses mWasabi under the control of CAG promoter, basic vector, multiclonig site at C terminal |
| 164162 | pCAG-mCherry2-G | Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal |
| 164163 | pCAG-mEGFP-HO-G | Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal |
| 164164 | pCAG-mCherry2-mito | Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter |
| 164165 | pCAG-mWasabi-ER | Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter |
| 164166 | pCAG-mCherry2-ER | Expresses mCherry2 fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter |
| 164167 | pAC-mCherry2-G4 | Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal |
| 164168 | pAC-mWasabi-G4 | Expresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal |
| 164169 | pAT-mCherry2-G | Expresses mCherry2 under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone |
| 164170 | pAT-mWasabi-G | Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone |