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Single-cell measurement of plasmid copy number and promoter activity.

Shao B, Rammohan J, Anderson DA, Alperovich N, Ross D, Voigt CA
Nat Commun. 2021 Mar 5;12(1):1475. doi: 10.1038/s41467-021-21734-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164122pSB207J23101 driving PP7 repeats
164123pSB208Ptac driving PP7 repeats
164125pSB219p15A backbone with PhlF binding sites
164127pSB223New measurement standard
164128pSB233PP7-CFP and PhlF-RFP expression plasmid
164130pSB235PhlF-RFP expression plasmid

Antibodies from Article