An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo.
Vallese F, Catoni C, Cieri D, Barazzuol L, Ramirez O, Calore V, Bonora M, Giamogante F, Pinton P, Brini M, Cali T
Nat Commun. 2020 Nov 27;11(1):6069. doi: 10.1038/s41467-020-19892-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164107 | SPLICS Mt-ER Long P2A | Detect the long Mitochondria-Endoplasmic reticulum contact |
| 164108 | SPLICS Mt-ER Short P2A | Detect the short Mitochondria-Endoplasmic reticulum contact |
| 164109 | SPLICS Mt-ER- PM Long P2A | Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact |
| 164110 | SPLICS Mt-ER- PM Short P2A | Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact |
| 164111 | SPLICS PM-ER Long P2A | Detect the long Plasma membrane-Endoplasmic reticulum contact |
| 164112 | SPLICS PM-ER Short P2A | Detect the short Plasma membrane-Endoplasmic reticulum contact |
| 164113 | SPLICS Po-ER Long P2A | Detect the long Peroxisomes-Endoplasmic reticulum contact |
| 164114 | SPLICS Po-ER Short P2A | Detect the long Peroxisomes-Endoplasmic reticulum contact |
| 164115 | SPLICS Po-Mt Long P2A | Detect the long Peroxisomes-mitochondria contact |
| 164116 | SPLICS Po-Mt Short P2A | Detect the short Peroxisomes-mitochondria contact |
| 164117 | SPLICS Mt-ER- Po Long P2A | Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact |
| 164118 | SPLICS Mt-ER- Po Short P2A | Detect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact |