Photocleavable proteins that undergo fast and efficient dissociation.
Lu X, Wen Y, Zhang S, Zhang W, Chen Y, Shen Y, Lemieux MJ, Campbell RE
Chem Sci. 2021 May 31;12(28):9658-9672. doi: 10.1039/d1sc01059j. eCollection 2021 Jul 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164033 | pET-PhoCl1-6His | Expressed PhoCl1 in a pET expression vector |
| 164034 | pBad-LgBiT-PhoCl1-SmBiT-MBP | luciferase-based PhoCl screening construct |
| 164035 | pBad-PhoCl2c | Improved PhoCl variant with enhanced dissociation efficiency |
| 164036 | pBad-PhoCl2f | Improved PhoCl variant with faster dissociation rate |
| 164037 | pcNDA-NES-PhoCl2c-mCherry | Optogenetic control of protein localization by PhoCl2c |
| 164048 | pcDNA-NES-PhoCl2f-mCherry | Optogenetic control of protein localization by PhoCl2f |
| 164049 | pcDNA-NBid-mMaple-CBid | Optogenetic manipulation of cell apoptosis negative control |
| 164050 | pcDNA-NBid-PhoCl1-CBid | Optogenetic manipulation of cell apoptosis using PhoCl1 |
| 164051 | pcDNA-NBid-PhoCl2c-CBid | Optogenetic manipulation of cell apoptosis using PhoCl2c |
| 164052 | pcDNA-NES-DEVD-mCardinal-NLS | Fluorescent reporter for caspase-3 |