A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae.
Dong C, Jiang L, Xu S, Huang L, Cai J, Lian J, Xu Z
ACS Synth Biol. 2020 Sep 18;9(9):2252-2257. doi: 10.1021/acssynbio.0c00218. Epub 2020 Aug 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163971 | pRS415-Cas9-VPR | Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast |
| 163972 | pRS423-SgH | Helper plasmid for cloning gRNAs under the control of the SNR52 promoter. |
| 163973 | pRS423-TyrSgH | Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter |
| 163974 | pRS423-ProSgH | Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter |