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A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila.

Harmansa S, Alborelli I, Bieli D, Caussinus E, Affolter M
Elife. 2017 Apr 11;6. doi: 10.7554/eLife.22549. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
163925pUASTLOTattB_vhhGFP4::Nrv1::tagBFPExpression of GrabFP-B-extracellular under control of UAS or LOP enhancer
163926pUASTLOTattB_mCherry::Nrv1::vhhGFP4Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer
163927pUASTLOTattB_vhhGFP4::T48-Baz::mCherryExpression of GrabFP-A-extracellular under control of UAS or LOP enhancer
163928pUASTLOTattB_mCherry::T48-Baz::vhhGFP4Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer
163929pUASTLOTattB_vhhGFP4::Vkg::mCherryExpression of GrabFP-ECM under control of UAS or LOP enhancer
163930pUASTLOTattB_mCherry-CD8-vhhGFPExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

Antibodies from Article