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In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1.

Duellberg C, Auer A, Canigova N, Loibl K, Loose M
Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2010054118. doi: 10.1073/pnas.2010054118. Epub 2020 Dec 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
163905ADAP1Full length mouse ADAP1 for purification from insect cells
163906mWasabi-ADAP1Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells
163907KIF13BFull length mouse KIF13B for purification from insect cells
163908KIF13B 1-584Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

Antibodies from Article